Chapter 2 Techniques in Cell Biology

Content of this chapter

Outline

1.The Light Microscopy

    A.Preparation of specimen
    B.Resolution and magnification
    C.Special Light Microscopes

2.Transmission Electron Microscopy
  
    A.The comparison of the lens systems of LM and TEM
    B.Specimen Preparation for Electron Microscopy


3.Scanning electron microscope (SEM)


4.The Fractionation and analysis for cell’s contents

    A.The technique of differential centrifugation
    B.Subsequent purification by Density-Gradient Equilibrium Centrifugation
    C.Isolation, purification, and fractionation of proteins
    D.Determining Protein-Protein Interaction
    E.Localization of a specific protein by immuno-electron microscopy
    F.Nucleic acid hybridization

5.Protein structure determination

     A.X-ray Crystallography
     B.Nuclear Magnetic Resonance (NMR) Spectroscopy
      
6.The techniques for cytochemistry

     A.Microspectrophotometry
     B.Flow Cytometry

7.Autoradiograph of LM and TEM

8.The Cell’s cultures and Cell’s engineering

     A.Primary culture cell and subculture cell
     B.Cell strain and Cell line      
     C.Cell’s engineering
     D.The technique for the take apart and gather up of cell, and microscope manipulation